Titrated extract of Centella asiatica increases hair inductive property through inhibition of STAT signaling pathway in three-dimensional spheroid cultured human dermal papilla cells
- PMID: 29032741
- DOI: 10.1080/09168451.2017.1385383
Titrated extract of Centella asiatica increases hair inductive property through inhibition of STAT signaling pathway in three-dimensional spheroid cultured human dermal papilla cells
Abstract
Dermal papilla (DP) is a pivotal part of hair follicle, and the smaller size of the DP is related with the hair loss. In this study, we investigated the effect of titrated extract of Centella asiatica (TECA) on hair growth inductive property on 3D spheroid cultured human DP cells (HDP cells). Significantly increased effect of TECA on cell viability was only shown in 3D sphered HPD cells, not in 2D cultured HDP cells. Also, TECA treatment increased the sphere size of HDP cells. The luciferase activity of STAT reporter genes and the expression of STAT-targeted genes, SOCS1 and SOCS3, were significantly decreased. Also, TECA treatment increased the expression of the hair growth-related signature genes in 3D sphered HDP cells. Furthermore, TECA led to downregulation of the level of phosphorylated STAT proteins in 3D sphered HDP cells. Overall, TECA activates the potential of hair inductive capacity in HDP cells.
Keywords: STAT; cell culture; dermal papilla cells; hair growth; titrated extract of Centella asiatica.
Similar articles
-
Flavonoid Silibinin Increases Hair-Inductive Property Via Akt and Wnt/β-Catenin Signaling Activation in 3-Dimensional-Spheroid Cultured Human Dermal Papilla Cells.J Microbiol Biotechnol. 2019 Feb 28;29(2):321-329. doi: 10.4014/jmb.1810.10050. J Microbiol Biotechnol. 2019. PMID: 30609881
-
Wnt activator CHIR99021-stimulated human dermal papilla spheroids contribute to hair follicle formation and production of reconstituted follicle-enriched human skin.Biochem Biophys Res Commun. 2019 Aug 27;516(3):599-605. doi: 10.1016/j.bbrc.2019.06.038. Epub 2019 Jun 18. Biochem Biophys Res Commun. 2019. PMID: 31221480
-
Surface Tension Guided Hanging-Drop: Producing Controllable 3D Spheroid of High-Passaged Human Dermal Papilla Cells and Forming Inductive Microtissues for Hair-Follicle Regeneration.ACS Appl Mater Interfaces. 2016 Mar 9;8(9):5906-16. doi: 10.1021/acsami.6b00202. Epub 2016 Feb 29. ACS Appl Mater Interfaces. 2016. PMID: 26886167
-
Monoterpenoid Loliolide Regulates Hair Follicle Inductivity of Human Dermal Papilla Cells by Activating the Akt/β-Catenin Signaling Pathway.J Microbiol Biotechnol. 2019 Nov 28;29(11):1830-1840. doi: 10.4014/jmb.1908.08018. J Microbiol Biotechnol. 2019. PMID: 31601058
-
TECA (Titrated Extract of Centella Asiatica): new microcirculatory, biomolecular, and vascular application in preventive and clinical medicine. A status paper.Panminerva Med. 2011 Sep;53(3 Suppl 1):105-18. Panminerva Med. 2011. PMID: 22108486 Review.
Cited by 2 articles
-
A Multimodal Hair-Loss Treatment Strategy Using a New Topical Phytoactive Formulation: A Report of Five Cases.Case Rep Dermatol Med. 2021 Feb 4;2021:6659943. doi: 10.1155/2021/6659943. eCollection 2021. Case Rep Dermatol Med. 2021. PMID: 33614172 Free PMC article.
-
Therapeutic Potential of Centella asiatica and Its Triterpenes: A Review.Front Pharmacol. 2020 Sep 4;11:568032. doi: 10.3389/fphar.2020.568032. eCollection 2020. Front Pharmacol. 2020. PMID: 33013406 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources